• 回答数

    3

  • 浏览数

    93

vera911213
首页 > 职称论文 > 关于酶的论文及其文献

3个回答 默认排序
  • 默认排序
  • 按时间排序

吃货终结者0416

已采纳

[1] Thoroson J S, Hoster T J, Jiang J, et al. Nature′s carbohydrate chemists: the enzymatic glycosylation of bioactive bacterial metabolites [J]. Curr Org Chem,2001,5(2):139[2] Weymouth?Wilson A C. The role of carbohydrates in biologically active natural products [J]. Nat Prod Rep,1997,14(2):99[3] Losey H C, Peczuh M W, Chen Z, et al. Tandem action of glycosyltransferases in the maturation of vancomycin and teicoplanin aglycones: novel glycopeptides [J]. Biochemistry,2001,40(15):4745[4] Cudic P, Kranz J K, Behenna D C, et al. Complexation of peptidoglycan intermediates by the lipoglycodepsipeptide antibiotic ramoplanin: minimal structural requirements for intermolecular complexation and fibril formation [J]. Proc Natl Acad Sci,2002,99(11):7384[5] Gellert M, O′Dea M H, Itoh T, et al. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase [J]. Proc Natl Acad Sci,1976,73(12):4474[6] Sosio M, Stinchi S, Beltrametti F, et al. The gene cluster for the biosynthesis of the glycopeptide antibiotic A40926 by nonomuraea species [J]. Chem Biol,2003,10(6):541[7] Quiros L M, Aguirrezabalaga I, Olano C, et al. Two glycosyltransferases and a glycosidase are involved in oleandomycin modification during its biosynthesis by Streptomyces antibioticus [J]. Mol Microbiol,1998,28(6):1177[8] Gourmelen A, Blondelet?Rouault M H, Pernodet J L. Characterization of a glycosyl transferase inactivating macrolides, encoded by gimA from Streptomyces ambofaciens [J]. Antimicrob Agents Chemother,1998,42(10):2612[9] 代焕琴. 安丝菌素生物合成的后修饰研究[D]. 中国科学院博士学位论文,2006:40[10] Walsh C T, Losey H C, Freel C L. Antibiotic glycosyltransferases [J]. Biochem Soc Trans,2003,31(Pt3):487[11] Lu C, Bai L, Shen Y. A novel amide N?glycoside of ansamitocins from Actinosynnema pretiosum [J]. J Antibiot,2004,57(5):348[12] Hoffmeister D, Ichinose K, Bechthold A. Two sequence elements of glycosyltransferases involved in urdamycin biosynthesis are responsible for substrate specificity and enzymatic activity [J]. Chem Biol,2001,8(16):557[13] Mulichak A M, Losey H C, Walsh C T, et al. Structure of the UDP?glucosyltransferase GtfB that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics [J]. Structure,2001,9(7):547[14] Sanchez C, ButovichI A, Brana A F, et al. The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives [J]. Chem Biol,2002,9(4):519[15] Otten S L, Liu X, Ferguson J, et al. Cloning and characterization of the Streptomyces peucetius dnrQS genes encoding a daunosamine biosynthesis enzyme and a glycosyltransferase involved in daunorubicin biosynthesis [J]. J Bacteriol,1995,177(22):6688[16] Zhao Y, Ahlert J, Xue Y, et al. Engineering a methy?mycin/pikromycin?calicheamicin hybrid: construction of two new macrolides carrying a designed sugar moiety [J]. J Am Chem Soc,1999,121(42):9881[17] Hoffmeister D, Ichinose K, Domann S, et al. The NDP?sugar co?substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster [J]. Chem Biol,2000,7(11):821[18] Walsh C, Freel C L, Losey H C. Antibiotic glycosyltransferases: antibiotic maturation and prospects for reprogramming [J]. J Med Chem,2003,46(16):3425[19] Blanco G, Patallo E P, Brana A F, et al. Identification of a sugar flexible glycosyltransferase from Streptomyces olivaceus: the producer of the antitumor polyketide elloramycin [J]. Chem Biol,2001,8(3):253[20] Dürr C, Hoffmeister D, Wohlert S E, et al. The glycosyltransferase UrdGT2 establishes both C? and O?glycosidic bonds [J]. Angewandte,2004,43(22):2962[21] Freel C L, Anderson J W, Kahne D, et al. Initial characterization of novobiocic acid noviosyl transferase activity of NovM in biosynthesis of the antibiotic novobiocin [J]. Biochemistry,2002,42(14):4179[22] Mendez C, Salas J A. Altering the glycosylation pattern of bioactive compounds [J]. Trends Biotechnol,2001,19(11):449[23] He X, M Liu, H W. Formation of unusual sugars: mechanistic studies and biosynthetic applications [J]. Annu Rev Biochem,2002,71:701[24] Oberthur M, Leimkuhler C, Kruger R G, et al. A systematic investigation of the synthetic utility of glycopeptide glycosyltransferases [J]. J Am Chem Soc,2005,127(30):10747[25] Wohlert S E, Blanco G, Lombo F, et al. Novel hybrid tetracenomycins through combinatorial biosynthesis using a glycosyltransferase encoded by elm genes in cosmid 16F4 and which shows a broad sugar substrate specificity [J]. J Am Chem Soc,1998,120(41):10596[26] Salas J A, Mendez C. Biosynthesis pathways for deoxysugars in antibiotic?producing actinomycetes: isolation, characterization and generation of novel glycosylated derivatives [J]. J Mol Microbiol Biotechnol,2005,9(2):77[27] Sanchez C, Zhu L, Brana A F, et al. Combinatorial biosynthesis of antitumor indolocarbazole compounds [J]. Proc Natl Acad Sci,2005,102(2):461[28] Salas A P, Zhu L, Sanchez C, et al. Deciphering the late steps in the biosynthesis of the anti?tumour indolocarbazole staurosporine: sugar donor substrate flexibility of the StaG glycosyltransferase [J]. Mol Microbiol,2005,58(1):17[29] Doumith M, Legrand R, Lang C, et al. Interspecies complementation in Saccharopolyspora erythraea: elucidation of the function of oleP1, oleG1 and oleG2 from the oleandomycin biosynthetic gene cluster of Streptomyces antibioticus and generation of new ery

355 评论

热心网友小王

留个邮箱啊,给你发过去

98 评论

犀牛望月0

在粮食陈化的过程中,过氧化氢酶的活性会降低,呼吸作用就减弱了;植酸酶,蛋白酶和磷脂酶活性等水解酶类都是会增加的。详细如下:粮食陈化中的有关变化1、生理变化粮食陈化的生理变化无论是含胚与不含胚的粮食主要表现为酶的活性和代谢水平的变化。粮食在储藏中,生理变化多是在各种酶的作用下进行的。若粮食中酶的活性减弱或丧失,其生理作用也随之而减弱或停止。随着陈化的进行粮食的生活力逐渐丧失,与呼吸有关的酶类,如过氧化氢酶的活性趋向降低,呼吸作用也随之减弱;而水解酶类,如植酸酶,蛋白酶和磷脂酶活性都增加。粮食在储藏中由于自身代谢的有毒产物积累也导致粮粒衰老和陈化,如吲哚乙酸和阿魏酸的积累和一些脂类氧化产物的积累都将加速粮食的陈化的进程。据报道,一些不饱和脂肪酸分解游离基与其它脂类起反应,能使细胞膜结构破坏。衰老的种子里,高尔基体散开并失水,溶酶体膜破裂,引起细胞的解体,同时细胞膜也丧失完整性而透性增强。对于有胚的粮食储藏中生理变化的指标是,随着陈化加深粮粒生活力与发芽率下降,随着细胞的劣变,细胞膜透性增强,浸出液所含的物质量增加,电导率增高。粮食陈化与酶活性的关系通常可以由一些与品质相关的酶活性变化加以反映。稻谷储藏初期含有活性较高的过氧化氢酶,淀粉酶,随着储藏时间的延长,这些酶的活性就大大减弱,生活力也下降。根据测定.稻谷储藏三年后过氧化氢酶活性降低五倍,淀粉酶等于零。大米在储藏中过氧化酶活性丧失,呼吸也趋于停止。现在人们测定粮食代谢水平,就采用过氧化氢酶的活性作为指标之一。 2、化学成分变化粮食化学成分的变化,无论含胚与不含胚的粮食,一般说多以脂肪变化较快,蛋白质其次,淀粉变化很微弱。脂肪的变化粮食储藏过程中,由于脂肪易于水解,游离脂肪酸在粮食中首先出现。特别是在环境条件适宜时,储粮霉菌开始繁殖,分泌出脂肪酶,参加脂肪水解,使粮食中脂肪酸增多,粮食陈化加深。蛋白质的变化粮食储藏过程中,受外界物理、生物等因素的影响,蛋白质的水解和变性。蛋白质水解后,游离氨基酸上升,酸度增加。蛋白质变性后,空间结构松散,肽键展延,非极性基外露,亲水基内藏,蛋白质由溶胶变为凝胶、溶解度降低,粮食陈化加深。淀粉的变化粮食储藏过程中,淀粉水解成的麦芽糖与糊精继续水解,还原糖增加,糊精相对减少,粘度下降,粮食开始陈化。 3、物理性质的变化粮食陈化时物理性质变化很大,表现为:粮粒组织硬化,柔性与韧性变弱,米质变脆,米粒起筋,身骨收缩,淀粉细胞变硬,细胞膜透性增强,糊化及吸水率降低,持水率亦降低,米饭破碎,粘性较差,口感有“陈味”。

157 评论

相关问答

  • 关于淀粉酶的研究论文

    Enzyme law preparation porous starch influence factor research. Abstract: The po

    怀疑本身 3人参与回答 2023-12-07
  • 教改及其相关研究论文

    1.论题大小应适中。遵照上文提到的可行性原则,论题不宜过大或过宽。否则,可能会出现两个问题:第一,可能超出作者本人的主观能力。特别对于刚刚从事教学工作的年轻教师

    家具加工批发 3人参与回答 2023-12-07
  • 文献综述格式及其论文

    毕业论文文献综述格式 文献综述是针对某一研究领域或专题搜集大量文献资料的基础上,就国内外在该领域或专题的主要研究成果、最新进展、研究动态、前沿问题等进行综合分析

    阳光通宝 3人参与回答 2023-12-05
  • 涉密学位论文及其相关资料

    研究生涉密学位论文需要向所在学校或者是科研院所的相关部门提交即可。 目前很多高校和科研院所都有一些涉密的科研项目,这些科研项目需要大量的人力支援进行参与该项工

    cotillardw 4人参与回答 2023-12-06
  • 关于机械制造及其自动化论文题目

    9YKG-2型高密度压捆机送料装置的设计小型玉米中耕机的设计实验室用小型自动换刀装置的设计小型马路清扫机的设计奶牛养殖场牛粪尿清理装罐发酵产沼气综合利用设计25

    地主李东家 3人参与回答 2023-12-09